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pcmv flag s127a yap  (Addgene inc)


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    Addgene inc pcmv flag s127a yap
    Pcmv Flag S127a Yap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+flag+s127a+yap/pCMV-flag+S127A+YAP+(Plasmid+%2327370)/bio_rxiv__2025__10__16__682743-68-14-15
    Average 93 stars, based on 36 article reviews
    pcmv flag s127a yap - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Loss of the wild-type KRAS allele promotes pancreatic cancer progression through functional activation of YAP1.
    Article Snippet: .. For transient transfections of plasmids HOP-FLASH (Addgene plasmid #833467), HIP-FLASH (Addgene plasmid#83466), pFLAG-YAP1 (Addgene plasmid #66853), and pCMV-flag S127A YAP (Addgene plasmid #27370), cell lines were grown to 90% confluence at the time of transfection. .. Lipofectamine 2000 (Invitrogen, Catalog #: 11668-027) and Opti-MEM® I Reduced Serum Medium (Catalog #: 31985-062) were used per manufacturer’s instruction.

    Plasmid Preparation:

    Article Title: Loss of the wild-type KRAS allele promotes pancreatic cancer progression through functional activation of YAP1.
    Article Snippet: .. For transient transfections of plasmids HOP-FLASH (Addgene plasmid #833467), HIP-FLASH (Addgene plasmid#83466), pFLAG-YAP1 (Addgene plasmid #66853), and pCMV-flag S127A YAP (Addgene plasmid #27370), cell lines were grown to 90% confluence at the time of transfection. .. Lipofectamine 2000 (Invitrogen, Catalog #: 11668-027) and Opti-MEM® I Reduced Serum Medium (Catalog #: 31985-062) were used per manufacturer’s instruction.

    Article Title: Plumbagin-loaded ZIF-90 nanoparticles suppress gastric cancer progression by targeting the YAP1 signaling
    Article Snippet: The YAP1 signaling pathway is not only an oncogenic signaling pathway but also an important therapeutic target in cancer.. Plumbagin (PLB) is a natural naphthoquinone that exhibits anticancer potential in diverse cancers.. However, the half-life of PLB in vivo is short.

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by Cells 2023, 12, 2121 3 of 20 subcloning mutant human YAPS127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370; RRID: Addgene_27370) [25], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/ addgene:19407; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    Article Title: The Hippo terminal effector YAP boosts enterovirus replication in type 1 diabetes
    Article Snippet: .. Following plasmids have been used: Kinase-dead (MST1-K59; dnMST1) was kindly provided by Dr. Junichi Sadoshima and Dr. Yasuhiro Maejima (UMDNJ, New Jersey Medical School). pCMV-Flag-YAP-S94A was a gift from Kunliang Guan (Addgene plasmid # 33102; http://n2t.net/addgene:33102 ; RRID: Addgene_33102) . pCEFL EGFP-TEADi was a gift from Ramiro Iglesias-Bartolome (Addgene plasmid # 140144; http://n2t.net/addgene:140144 ; RRID: Addgene_140144) . pCMV-flag S127A YAP was a gift from Kunliang Guan (Addgene plasmid # 27370; http://n2t.net/addgene:27370 ; RRID: Addgene_27370) . ..

    Article Title: The Hippo terminal effector YAP boosts enterovirus replication in type 1 diabetes.
    Article Snippet: .. Following plasmids have been used: Kinase-dead (MST1-K59; dnMST1) was kindly provided by Dr. Junichi Sadoshima and Dr. Yasuhiro Maejima (UMDNJ, New Jersey Medical School). pCMV-Flag-YAPS94Awas a gift fromKunliang Guan (Addgene plasmid # 33102; http:// n2t.net/addgene:33102; RRID: Addgene_33102)41. pCEFL EGFP-TEADi was a gift fromRamiro Iglesias-Bartolome (Addgene plasmid # 140144; http://n2t.net/addgene:140144; RRID: Addgene_140144)68. pCMV-flag S127A YAP was a gift from Kunliang Guan (Addgene plasmid # 27370; http://n2t.net/addgene:27370; RRID: Addgene_27370)129. ..

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by subcloning mutant human YAP S127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370 ; RRID: Addgene_27370) [ ], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/addgene:19407 ; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    Over Expression:

    Article Title: Wild-type and mutated ß-catenin differently repress RND3/RHOE expression in hepatocellular carcinoma
    Article Snippet: .. For overexpression of YAP, plasmids were obtained from Addgene: pcDNA-Flag-Yap1 (addgene#18881; ( )) and pCMV-flag-S127A-YAP (addgene#27370; ( )) encodes respectively, WT or constitutively activated human Yap1. .. DNA transfections were performed in HepG2 cells using Lipofectamine 3000 transfection reagent (ThermoFischer) according to manufacturer’s protocol.

    Generated:

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by Cells 2023, 12, 2121 3 of 20 subcloning mutant human YAPS127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370; RRID: Addgene_27370) [25], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/ addgene:19407; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by subcloning mutant human YAP S127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370 ; RRID: Addgene_27370) [ ], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/addgene:19407 ; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    Subcloning:

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by Cells 2023, 12, 2121 3 of 20 subcloning mutant human YAPS127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370; RRID: Addgene_27370) [25], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/ addgene:19407; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by subcloning mutant human YAP S127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370 ; RRID: Addgene_27370) [ ], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/addgene:19407 ; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    Mutagenesis:

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by Cells 2023, 12, 2121 3 of 20 subcloning mutant human YAPS127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370; RRID: Addgene_27370) [25], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/ addgene:19407; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
    Article Snippet: .. AdTRE S127A YAP adenoviral vector was generated by subcloning mutant human YAP S127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370 ; RRID: Addgene_27370) [ ], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/addgene:19407 ; RRID:Addgene_19407). .. The resulting TRE-S127A YAP fragment was cloned to pENTR11 shuttle vector (Invitrogen) to enable the generation of adenovirus construct (AdTRE-S127A YAP) using the Gateway system (Invitrogen, Warrington, UK).

    other:

    Article Title: C12orf59 Promotes Esophageal Squamous Cell Carcinoma Progression via YAP-Mediated Epithelial-Mesenchymal Transition
    Article Snippet: Constitutively active (CA)-YAP (pCMV-Flag S127A YAP; Addgene plasmid #27370) was a gift from Kunliang Guan.



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    Figure 3. Doxycycline-induced <t>YAPS127A</t> expression increases YAP activity in iPSC-CMs. (A) YAP activity determined by luciferase reporter assay in hiPSC-CMs following transduction with TRE- YAPS127A + rtTA adenovirus, and YAP induction with 50–1000 ng/mL doxycycline. Statistical significance determined by one-way ANOVA followed by Tukey’s post hoc multiple comparison. n = 8 replicates per group, **** p < 0.0001. (B) Western Blot demonstrating overexpression of YAP and active YAP in hiPSC-CMs following YAPS127A transduction and induction with 50–1000 ng/mL doxycycline. (C–H) qRT-PCR analysis of transcript levels of YAP targets Birc5, Tead1, Ankrd1, Pikcb3, Fgf2, and Cyr61 relative to housekeeping gene Gapdh 7 days following doxycycline-induced YAPS127A
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    Image Search Results


    Figure 3. Doxycycline-induced YAPS127A expression increases YAP activity in iPSC-CMs. (A) YAP activity determined by luciferase reporter assay in hiPSC-CMs following transduction with TRE- YAPS127A + rtTA adenovirus, and YAP induction with 50–1000 ng/mL doxycycline. Statistical significance determined by one-way ANOVA followed by Tukey’s post hoc multiple comparison. n = 8 replicates per group, **** p < 0.0001. (B) Western Blot demonstrating overexpression of YAP and active YAP in hiPSC-CMs following YAPS127A transduction and induction with 50–1000 ng/mL doxycycline. (C–H) qRT-PCR analysis of transcript levels of YAP targets Birc5, Tead1, Ankrd1, Pikcb3, Fgf2, and Cyr61 relative to housekeeping gene Gapdh 7 days following doxycycline-induced YAPS127A

    Journal: Cells

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

    doi: 10.3390/cells12172121

    Figure Lengend Snippet: Figure 3. Doxycycline-induced YAPS127A expression increases YAP activity in iPSC-CMs. (A) YAP activity determined by luciferase reporter assay in hiPSC-CMs following transduction with TRE- YAPS127A + rtTA adenovirus, and YAP induction with 50–1000 ng/mL doxycycline. Statistical significance determined by one-way ANOVA followed by Tukey’s post hoc multiple comparison. n = 8 replicates per group, **** p < 0.0001. (B) Western Blot demonstrating overexpression of YAP and active YAP in hiPSC-CMs following YAPS127A transduction and induction with 50–1000 ng/mL doxycycline. (C–H) qRT-PCR analysis of transcript levels of YAP targets Birc5, Tead1, Ankrd1, Pikcb3, Fgf2, and Cyr61 relative to housekeeping gene Gapdh 7 days following doxycycline-induced YAPS127A

    Article Snippet: AdTRE S127A YAP adenoviral vector was generated by Cells 2023, 12, 2121 3 of 20 subcloning mutant human YAPS127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370; RRID: Addgene_27370) [25], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/ addgene:19407; RRID:Addgene_19407).

    Techniques: Expressing, Activity Assay, Luciferase, Reporter Assay, Transduction, Comparison, Western Blot, Over Expression, Quantitative RT-PCR

    Figure 4. Doxycycline-induced YAPS127A expression enhances iPSC-CM proliferation and survival. Immunocytochemistry analysis of proliferation markers 24 h following doxycycline-induced YAPS127A

    Journal: Cells

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

    doi: 10.3390/cells12172121

    Figure Lengend Snippet: Figure 4. Doxycycline-induced YAPS127A expression enhances iPSC-CM proliferation and survival. Immunocytochemistry analysis of proliferation markers 24 h following doxycycline-induced YAPS127A

    Article Snippet: AdTRE S127A YAP adenoviral vector was generated by Cells 2023, 12, 2121 3 of 20 subcloning mutant human YAPS127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370; RRID: Addgene_27370) [25], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/ addgene:19407; RRID:Addgene_19407).

    Techniques: Expressing, Immunocytochemistry

    Figure 5. iPSC-CM maturation and morphology following doxycycline-induced YAPS127A expression. (A–F) qRT-PCR analysis of transcript levels of cardiomyocyte maturation markers RyR, SERCA2A, Myh6, Myh7, Myl2, and Cx43 relative to housekeeping gene Gapdh 7 days following doxycycline- induced YAPS127A overexpression. Statistical significance determined by unpaired t-test. N = 6 independent experiments, with n = 9–12 replicates in each group, * p < 0.05, ** p < 0.01, **** p < 0.0001. (G–J) iPSC-CMs fixed and stained with phalloidin (green) 7 days following doxycycline-induced YAPS127A overexpression for analysis of cell perimeter, cross-sectional area and circularity. Scale bars represent 20 µM. Statistical significance determined by unpaired t-test. n = 25–30 replicates per group. ** p < 0.01, **** p < 0.0001.

    Journal: Cells

    Article Title: Genetic and Pharmacological YAP Activation Induces Proliferation and Improves Survival in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

    doi: 10.3390/cells12172121

    Figure Lengend Snippet: Figure 5. iPSC-CM maturation and morphology following doxycycline-induced YAPS127A expression. (A–F) qRT-PCR analysis of transcript levels of cardiomyocyte maturation markers RyR, SERCA2A, Myh6, Myh7, Myl2, and Cx43 relative to housekeeping gene Gapdh 7 days following doxycycline- induced YAPS127A overexpression. Statistical significance determined by unpaired t-test. N = 6 independent experiments, with n = 9–12 replicates in each group, * p < 0.05, ** p < 0.01, **** p < 0.0001. (G–J) iPSC-CMs fixed and stained with phalloidin (green) 7 days following doxycycline-induced YAPS127A overexpression for analysis of cell perimeter, cross-sectional area and circularity. Scale bars represent 20 µM. Statistical significance determined by unpaired t-test. n = 25–30 replicates per group. ** p < 0.01, **** p < 0.0001.

    Article Snippet: AdTRE S127A YAP adenoviral vector was generated by Cells 2023, 12, 2121 3 of 20 subcloning mutant human YAPS127A cDNA from the pCMV-flag S127A YAP, which was a gift from Kunliang Guan (Addgene plasmid #27370; http://n2t.net/addgene:27370; RRID: Addgene_27370) [25], into pTREtight2 vector backbone containing tight TRE promoter (pTREtight2 was a gift from Markus Ralser, Addgene plasmid #19407; http://n2t.net/ addgene:19407; RRID:Addgene_19407).

    Techniques: Expressing, Quantitative RT-PCR, Over Expression, Staining